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KMID : 0604520050310020141
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 2 p.141 ~ p.146
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Development of Chitosan Coated Solid Lipid Nano-particles Containing 7-Dehydrocholesterol
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Lee Geun-Soo
Kim Tae-Hoon
Lee Cheon-Il
Pyo Hyeong-Bae
Choe Tae-Boo
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Abstract
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Unstable cosmetic active ingredients could rapidly break down in chemical and photochemical process. Therefore, it has become a very important issue to encapsulate active ingredient for the stabilization. 7-Dehydrocholesterol (7-DHC), a precursor of vitamin D_3, has been shown to increase levels of protein and mRNA for heat shock protein in normal human epidermal keratinocytes. However, topical dermal application of 7-DHC is restricted due to its poor solubility and chemical unstability. In this study, 7-DHC was incorporated into nano-emulsion (NE), solid lipid nano-particle (SLN), and chitosan coated solid lipid nano-particle (CASLN), respectively. In order to prepare NE and SLN dispersion, high-pressure homogenization at temperature above the melting point of lipid was used Hydrogenated lecithin and polysorbate 60 were used as stabilizer for NE and SLN. CASLN was prepared by high speed homogenizing after adding chitosan solution to the SLN dispersion and showed positively charged particle properties. Decomposition rate of 7-DHC in NE, SLN and CASLN was studied as a function of time at different temperature. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) studies were performed to characterize state of lipid modification. It appeared that CASLN is the most effective to stabilize 7-DHC and may be used for a useful topical dermal delivery system.
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KEYWORD
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7-dehydrocholesterol (7-DHC), chitosan coated solid lipid nanoparticle (CASLN), hyrogenated lecithin, career, long term stability
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